Composite

Part:BBa_K3040009:Design

Designed by: TSAI, CHIA-TSEN   Group: iGEM19_NTHU_Taiwan   (2019-10-18)


rrnD promoter with TP24 UP element and a fadR binding site inserted between spacer region regulating


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 705
    Illegal AgeI site found at 817
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PRESNELL, Kristin V.; FLEXER-HARRISON, Madeleine; ALPER, Hal S. Design and synthesis of synthetic UP elements for modulation of gene expression in Escherichia coli. Synthetic and systems biotechnology, 2019, 4.2: 99-106.


Source

PRESNELL, Kristin V.; FLEXER-HARRISON, Madeleine; ALPER, Hal S. Design and synthesis of synthetic UP elements for modulation of gene expression in Escherichia coli. Synthetic and systems biotechnology, 2019, 4.2: 99-106.

References